Fiche chercheur

Fabrice Neiers

Phone : +33 (0)3 80 69 32 02

Email : fabrice.neiers@u-bourgogne.fr

Address : CSGA INRAE 17 rue Sully 21065 Dijon FRANCE

Team "Sugars-Sweet Perception and Receptors "

Research topic Skills Recent publications

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Presentation

My research focus on the understanding of the biochemistry of the smell and taste perception.

Education

PhD in molecular enzymology at Lorraine University under the supervision of Pr. Guy Branlant.

Postodoctoral position in Karolinska Institut in two laboratories: the Brigitta and Staffan Normark laboratory, and the Adnane Achour laboratory.

Professional experience

From 2009, Ass. Professor in the Medical/Pharmaceutical school of the Burgundy University.

Research topic

I develop three axes focusing on the understanding of the perception of smell and taste at the molecular level. Axe 1: Caracterisation of the human proteins involved in the taste and smell modulation. Axe 2: Decephering the actif site modifications of the Drosophila Glutathione transferase enzymes during their evolution in link with their role in the taste and smell perception. Axe 3: Development of new microbiological organism for the recombinant protein production.

Skills

Enzymology, Recombinant protein production and purification, X-ray crystallography, Microbiology, Molecular biology, Biophysical analysis (Circular dichroïsm, Light scattering, Microcalorimetry, Fluorescence spectroscopy).

Recent publications

Biophysical and functional characterization of the human TAS1R2 sweet taste receptor overexpressed in a HEK293S inducible cell line. Belloir C, Brulé M, Tornier L, Neiers F, Briand L. Sci Rep. 2021 Nov 15;11(1):22238. doi: 10.1038/s41598-021-01731-3. PMID: 34782704 Free PMC article.

Oral enzymatic detoxification system: Insights obtained from proteome analysis to understand its potential impact on aroma metabolization. Schwartz M, Neiers F, Charles JP, Heydel JM, Muñoz-González C, Feron G, Canon F. Compr Rev Food Sci Food Saf. 2021 Nov;20(6):5516-5547. doi: 10.1111/1541-4337.12857. Epub 2021 Oct 15. PMID: 34653315 Free article. Review.

Effects of oenological tannins on aroma release and perception of oxidized and non-oxidized red wine: A dynamic real-time in-vivo study coupling sensory evaluation and analytical chemistry. Pittari E, Piombino P, Andriot I, Cheynier V, Cordelle S, Feron G, Gourrat K, Le Quéré JL, Meudec E, Moio L, Neiers F, Schlich P, Canon F. Food Chem. 2022 Mar 15;372:131229. doi: 10.1016/j.foodchem.2021.131229. Epub 2021 Sep 27. PMID: 34624784

Impact of Oral Microbiota on Flavor Perception: From Food Processing to In-Mouth Metabolization. Schwartz M, Canon F, Feron G, Neiers F, Gamero A. Foods. 2021 Aug 26;10(9):2006. doi: 10.3390/foods10092006. PMID: 34574116 Free PMC article. Review.

Adaptive Variation of Buchnera Endosymbiont Density in Aphid Host Acyrthosiphon pisum Controlled by Environmental Conditions. Neiers F, Saliou JM, Briand L, Robichon A. ACS Omega. 2021 Jul 6;6(28):17902-17914. doi: 10.1021/acsomega.1c01465. eCollection 2021 Jul 20. PMID: 34308025

Lactobacillus stress protein GroEL prevents colonic inflammation. Dias AMM, Douhard R, Hermetet F, Regimbeau M, Lopez TE, Gonzalez D, Masson S, Marcion G, Chaumonnot K, Uyanik B, Causse SZ, Rieu A, Hadi T, Basset C, Chluba J, Grober J, Guzzo J, Neiers F, Ortega-Deballon P, Demidov ON, Lirussi F, Garrido C. J Gastroenterol. 2021 May;56(5):442-455. doi: 10.1007/s00535-021-01774-3. Epub 2021 Mar 29. PMID: 33782752

The odorant metabolizing enzyme UGT2A1: Immunolocalization and impact of the modulation of its activity on the olfactory response. Neiers F, Jarriault D, Menetrier F, Briand L, Heydel JM. PLoS One. 2021 Mar 25;16(3):e0249029. doi: 10.1371/journal.pone.0249029. eCollection 2021. PMID: 33765098 Free PMC article.

Canon F, Belloir C, Bourillot E, Brignot H, Briand L, Feron G, Lesniewska E, Nivet C, Septier C, Schwartz M, Tournier C, Vargiolu R, Wang M, Zahouani H, Neiers F. J Agric Food Chem. 2021 Apr 7;69(13):3822-3826. doi: 10.1021/acs.jafc.0c07474. Epub 2021 Mar 8. PMID: 33682421

The Relationship Between Salivary Redox, Diet, and Food Flavor Perception. Schwartz M, Neiers F, Feron G, Canon F. Front Nutr. 2021 Jan 28;7:612735. doi: 10.3389/fnut.2020.612735. eCollection 2020. PMID: 33585536 Free PMC article. Review.

Nanofitins targeting heat shock protein 110: An innovative immunotherapeutic modality in cancer. Marcion G, Hermetet F, Neiers F, Uyanik B, Dondaine L, Dias AMM, Da Costa L, Moreau M, Bellaye PS, Collin B, Gobbo J, Briand L, Seigneuric R, Kitten O, Cinier M, Garrido C. Int J Cancer. 2021 Jun 15;148(12):3019-3031. doi: 10.1002/ijc.33485. Epub 2021 Mar 4. PMID: 33506516

Comparison of Different Signal Peptides for the Efficient Secretion of the Sweet-Tasting Plant Protein Brazzein in Pichia pastoris. Neiers F, Belloir C, Poirier N, Naumer C, Krohn M, Briand L. Life (Basel). 2021 Jan 13;11(1):46. doi: 10.3390/life11010046. PMID: 33450886 Free PMC article.

The association between changes of gustatory function and changes of salivary parameters: A pilot study. Zhu Y, Feron G, Von Koskull D, Neiers F, Brignot H, Hummel T. Clin Otolaryngol. 2021 May;46(3):538-545. doi: 10.1111/coa.13705. Epub 2021 Jan 21. PMID: 33370507

Lipoproteins LDL versus HDL as nanocarriers to target either cancer cells or macrophages. Hadi T, Ramseyer C, Gautier T, Bellaye PS, Lopez T, Schmitt A, Foley S, Yesylevskyy S, Minervini T, Douhard R, Dondaine L, Proukhnitzky L, Messaoudi S, Wendremaire M, Moreau M, Neiers F, Collin B, Denat F, Lagrost L, Garrido C, Lirussi F.JCI Insight. 2020 Dec 17;5(24):e140280. doi: 10.1172/jci.insight.140280.PMID: 33252359 Free PMC article.

Bacterial expression and purification of vertebrate odorant-binding proteins. Brulé M, Glaz M, Belloir C, Poirier N, Moitrier L, Neiers F, Briand L.Methods Enzymol. 2020;642:125-150. doi: 10.1016/bs.mie.2020.05.002. Epub 2020 May 30.PMID: 32828250

Interactions Between Odorants and Glutathione Transferases in the Human Olfactory Cleft. Schwartz M, Menetrier F, Heydel JM, Chavanne E, Faure P, Labrousse M, Lirussi F, Canon F, Mannervik B, Briand L, Neiers F. Chem Senses. 2020 Nov 7;45(8):645-654. doi: 10.1093/chemse/bjaa055.PMID: 32822468

Curvature and Torsion of Protein Main Chain as Local Order Parameters of Protein Unfolding. Grassein P, Delarue P, Nicolaï A, Neiers F, Scheraga HA, Maisuradze GG, Senet P.J Phys Chem B. 2020 Jun 4;124(22):4391-4398. doi: 10.1021/acs.jpcb.0c01230. Epub 2020 May 24.PMID: 32392067

Macrophage-induced reactive oxygen species promote myometrial contraction and labor-associated mechanisms†. Wendremaire M, Hadi T, Pezze M, Barrichon M, Lopez T, Neiers F, Sagot P, Garrido C, Lirussi F.Biol Reprod. 2020 May 26;102(6):1326-1339. doi: 10.1093/biolre/ioaa032.PMID: 32167534

Modulation of Sex Pheromone Discrimination by A UDP-Glycosyltransferase in Drosophila melanogaster. Fraichard S, Legendre A, Lucas P, Chauvel I, Faure P, Neiers F, Artur Y, Briand L, Ferveur JF, Heydel JM.Genes (Basel). 2020 Feb 25;11(3):237. doi: 10.3390/genes11030237.PMID: 32106439 Free PMC article.

Structure and steroid isomerase activity of Drosophila glutathione transferase E14 essential for ecdysteroid biosynthesis. Škerlová J, Lindström H, Gonis E, Sjödin B, Neiers F, Stenmark P, Mannervik B.FEBS Lett. 2020 Apr;594(7):1187-1195. doi: 10.1002/1873-3468.13718. Epub 2020 Jan 9.PMID: 31845319

Collisional mechanism of ligand release by Bombyxmori JHBP, a member of the TULIP / Takeout family of lipid transporters. Dupas S, Neiers F, Granon E, Rougeux E, Dupont S, Beney L, Bousquet F, Shaik HA, Briand L, Wojtasek H, Charles JP.Insect Biochem Mol Biol. 2020 Feb;117:103293. doi: 10.1016/j.ibmb.2019.103293. Epub 2019 Dec 3.PMID: 31809784

A conserved odorant binding protein is required for essential amino acid detection in Drosophila. Rihani K, Fraichard S, Chauvel I, Poirier N, Delompré T, Neiers F, Tanimura T, Ferveur JF, Briand L.Commun Biol. 2019 Nov 22;2:425. doi: 10.1038/s42003-019-0673-2. eCollection 2019.PMID: 31799428 Free PMC article.

The Drosophila odorant-binding protein 28a is involved in the detection of the floral odour ß-ionone. Gonzalez D, Rihani K, Neiers F, Poirier N, Fraichard S, Gotthard G, Chertemps T, Maïbèche M, Ferveur JF, Briand L.Cell Mol Life Sci. 2019 Sep 28. doi: 10.1007/s00018-019-03300-4. Online ahead of print.PMID: 31564000 Cite

Plant polyphenols, chemoreception, taste receptors and taste management. Canivenc-Lavier MC, Neiers F, Briand L.Curr Opin Clin Nutr Metab Care. 2019 Nov;22(6):472-478. doi: 10.1097/MCO.0000000000000595.PMID: 31490201 Cite

Characterization of rat glutathione transferases in olfactory epithelium and mucus. Heydel JM, Menetrier F, Belloir C, Canon F, Faure P, Lirussi F, Chavanne E, Saliou JM, Artur Y, Canivenc-Lavier MC, Briand L, Neiers F .PLoS One. 2019 Jul 24;14(7):e0220259. doi: 10.1371/journal.pone.0220259. eCollection 2019.PMID: 31339957 Free PMC article.

Nasal odorant metabolism: enzymes, activity and function in olfaction. Heydel JM, Faure P, Neiers F. Drug Metab Rev. 2019 May;51(2):224-245. doi: 10.1080/03602532.2019.1632890. Epub 2019 Jul 9.PMID: 31203698 Review.

Publisher Correction: Nasal mucus glutathione transferase activity and impact on olfactory perception and neonatal behavior. Robert-Hazotte A, Faure P, Neiers F, Potin C, Artur Y, Coureaud G, Heydel JM.Sci Rep. 2019 May 28;9(1):8111. doi: 10.1038/s41598-019-44135-0.PMID: 31138839 Free PMC article.

Selecting the first chemical molecule inhibitor of HSP110 for colorectal cancer therapy. Gozzi GJ, Gonzalez D, Boudesco C, Dias AMM, Gotthard G, Uyanik B, Dondaine L, Marcion G, Hermetet F, Denis C, Hardy L, Suzanne P, Douhard R, Jego G, Dubrez L, Demidov ON, Neiers F, Briand L, Sopková-de Oliveira Santos J, Voisin-Chiret AS, Garrido C.Cell Death Differ. 2020 Jan;27(1):117-129. doi: 10.1038/s41418-019-0343-4. Epub 2019 May 8.PMID: 31068676

Nasal mucus glutathione transferase activity and impact on olfactory perception and neonatal behavior. Robert-Hazotte A, Faure P, Neiers F, Potin C, Artur Y, Coureaud G, Heydel JM.Sci Rep. 2019 Feb 28;9(1):3104. doi: 10.1038/s41598-019-39495-6.PMID: 30816217 Free PMC article.

Data on the expression of GSTE1 and GSTE7 in Drosophila chemosensory organs after isothiocyanate exposure. Data Brief. 2018. Fraichard S., Gonzalez D., Grassein P., Delarue P., Senet P., Nicolaï A., Chavanne E., Mucher E., Artur Y., Ferveur J.F., Heydel J.M., Briand L., Neiers F. (IF : 0.3).

The Crystal Structure of Gurmarin, a Sweet Taste-Suppressing Protein: Identification of the Amino Acid Residues Essential for Inhibition. Chem Senses. 2018. Sigoillot M., Brockhoff A., Neiers F., Poirier N., Belloir C., Legrand P., Charron C., Roblin P., Meyerhof W., Briand L. (IF : 3.2).

Saliva and flavour perception: perspectives. J Agric Food Chem. 2018. Canon F., Neiers F., Guichard E. (IF : 2.9).

Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification. Insect Biochem Mol Biol. 2018 Gonzalez D., Fraichard S., Grassein P., Delarue P., Senet P., Nicolaï A., Chavanne E., Mucher E., Artur Y., Ferveur J.F., Heydel J.M., Briand L., Neiers F.

Effect of high pressure on the antimicrobial activity and secondary structure of the bacteriocin nisin. Innovative Food Science & Emerging Technologies. 2018. Modugnoa C., Loupiac C., Bernarda A., Jossiera A., Neiers F., Perrier-Cornet J.M., Simonin H. (IF : 1.2).

Biophysical and functional characterization of the N-terminal domain of the cat T1R1 umami taste receptor expressed in Escherichia coli. PLoS One. 2017. Belloir C., Savistchenko J., Neiers F., Taylor A.J., McGrane S., Briand L. (IF : 2.8).

A self-inducible heterologous protein expression system in Escherichia coli. Sci Rep. 2016 Briand L., Marcion G., Kriznik A., Heydel J.M., Artur Y., Garrido C., Seigneuric R., Neiers F.

What Does Diabetes "Taste" Like? Curr Diab Rep. 2016 Neiers F, Canivenc-Lavier M.C., Briand L.

Binding of Divalent Cations to Polygalacturonate: A Mechanism Driven by the Hydration Water. J Phys Chem B. 2016 (IF : 3.2) Huynh U.T., Lerbret A., Neiers F., Chambin O., Assifaoui A.

PhD surpervision

Anni Laffitte, PhD in 2017.

Christine Belloir, PhD in 2019.

Margot Glaz and Elodie Gonis both in thesis.

Additional responsibilities